- PRODUCT DESCRIPTION
+ The most common known genetic risk factor of venous thrombosis is resistance to activated C,
+ Which in the majority of cases is due to a single guanine-to- adenine nucleotide point mutation in exon 10 of the Factor V gene.
+ The result is an arginine replacement, at amino position 506, with glutamine (Factor V Arg506Gln or Factor V Leiden).
+ This mutation is associated with an increased risk of deep vein thrombosis.
- PRINCIPLE OF THE SYSTEM
+ During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end,
+ Separates the reporter dye from the quencer dye only when the probe hybridizes perfectly to the target DNA.
+ This cleavage results in the fluorescent signal which is monitored by Real-Time PCR detection system.
+ An increase in the fluorescent signal (CT) is proportional to the amount of the specific PCR product.
